Results can be displayed by several methods, of which the simplest is to calculate the final concentrations by multiplying by the appropriate dilution factor and plotting these against dilution. Dilutions are often made serially, although this is not the best approach as any errors made with each dilution are compounded. Several samples should be assessed, and the initial samples and dilutions chosen to fall evenly across the working range of the assay. Parallelism is assessed by assaying samples diluted in an appropriate analyte-free matrix. It provides information that is of more practical benefit than recovery as it answers the question: if a sample is diluted will it give the same result (when corrected for the dilution)-and if not, which is the most accurate result? Dilution experiments are often referred to as parallelism studies because effectively one is judging whether or not dilutions of sample lie parallel to the calibration curve. Chris Davies, in The Immunoassay Handbook (Fourth Edition), 2013 DilutionÄilution is a useful additional check on the accuracy of an assay.
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